Antibacteria activity of mango leaf extract on Bacillus sp and Escherichia coli Department:Microbiology By: Ziggerzillion Project ID: 7544 Rating: (5.0) votes: 1 Price:₦3000 Get the Complete MaterialAbstractThe active components of leaves of Mangifera indica L. were extracted using cold water and organic solvents (acetone and methanol) and were tested against Staphylococcus aureus, Streptococcus pyogenase, Streptococcus pneumoniae, Bacillus cereus, Escherichia coli, Pseudomonas aerugenosa, Proteus mirabilis, Salmonella typhi and Shigella flexnerri using the agar well (cup plate) diffusion method. Both the acetone and methanol extracts inhibited the growth of gram positive bacteria, with acetone extract exerting more activities on all the gram positive bacteria with zone of inhibition between 15 - 16 mm, and a gram negative bacterium S. typhi (14 mm) at 250 mg/ml. Whereas, water extract was not active on any of the bacterial pathogens tested at any of the concentration of the extract used. The activities of the plant extracts on the inhibited pathogens using the zone of inhibition were not as effective as the standard commercial antibacterial disks of gentamicin and erythromycin (t 2.23, p 0.05). Increased temperature (60 and 100°C for 1 h) had a multiplier effect on the activity of the extracts, but alkaline pH decreased the activity. Preliminary phytochemical analysis revealed the presence of tannins, glycosides, saponins and phenols. The MIC and MBC of the extracts was in the range of 12.5 - 75 and 25 - 175 mg/ml respectively. There is a basis for the traditional use of the plant as a local health remedy. ...Preview Download Preview +Other Microbiology project topics and materials you might be interested in»Isolation and identification of microbes associated with spoilage bread»Bacterial analysis of urine polluted environment in federal polytechnic Nekede, Owerri»Microbiological quality of palm kernel fruits»Microbiological evaluation of home made grape juice»Therapeutic potential of Moringa Oleifera leaves»Antibacterial activity of honey on staphylococcus aureusescherichia coli and streptococcus pyogen isolated from wound»Phytochemical screening and antimicrobial assessment of Spondias Mombin (Ijikara)»Bacteriological monitoring of abattoirs in Ehime Mbano in Imo state»Biodeterioration of natural and artifical stone»Proximate, selected vitamins, and minerals analysis of Moringa Oleifera»The effect of crude avocado and pawpaw leaves extract on kidney function of guinea pigs»Microbial examination of raw meat obtained at main abattoir, Offa»Isolation and identification of bacterial aerosols bioaerosols dump sites in Yenagoa»Isolation and characterization of fungi associated with the spoilage of corn grown in farms in Rukpokwu community»Incidence of staphylococcus and salmonella species in Kunuaya retailed in Yenagoa