Evaluation of antimicrobial effect of natural compounds (blue vitriol, brimstone and snake stone) on dental caries isolates Department:Microbiology By: PRINDYTECHNIE Project ID: 8857 Rating: (5.0) votes: 1 Price:₦4000 Get the Complete MaterialAbstractNatural compounds are chemical compounds or substances produced by a living organism or found in nature. The natural compounds of interest are sulphur stones, blue stones, and black stones. Dental caries is the localized destruction of susceptible dental hard tissues by acidic by-products from bacterial fermentation of dietary carbohydrates. This research work is aimed at evaluating the antimicrobial effect of selected natural compounds on microorganisms implicated in dental caries. Specific objectives include isolating and identifying the microorganisms present in dental caries and ascertaining the antimicrobial effects of natural compounds on these microorganisms. This project work was carried out at the Microbiology Laboratory Unit, Nnamdi Azikiwe University, Awka. Thirty dental swab specimens were carefully and aseptically collected individually from dental caries patients. Plates of nutrient and Sabouraud’s dextrose agar were inoculated using the spread plate method and incubated at 25 °C for 24hours and 48 hours, respectively. The isolates were identified and characterized based on their microscopic, macroscopic, biochemical and physiological characteristics. The identification tests include Gram staining, catalase, coagulase, motility, citrate, hemolysis and sugar fermentation tests. The pure cultures isolated include Staphylococcus sp., Streptococcus sp., and Bacillus sp. (bacteria) and Candida sp. (fungus). For antimicrobial susceptibility of the natural compounds, the agar well diffusion method was used. The test showed that the isolates were susceptible to some of the natural compounds as observed in the MIC, MBC and IZD values. The findings made from this research work showed that natural compounds can be used as antimicrobials and their production at an industrial level for dental caries treatment is encouraged. ...Preview Download Preview +Other Microbiology project topics and materials you might be interested in»Isolation and identification of microbes associated with spoilage bread»Bacterial analysis of urine polluted environment in federal polytechnic Nekede, Owerri»Microbiological quality of palm kernel fruits»Microbiological evaluation of home made grape juice»Therapeutic potential of Moringa Oleifera leaves»Antibacterial activity of honey on staphylococcus aureusescherichia coli and streptococcus pyogen isolated from wound»Phytochemical screening and antimicrobial assessment of Spondias Mombin (Ijikara)»Bacteriological monitoring of abattoirs in Ehime Mbano in Imo state»Proximate, selected vitamins, and minerals analysis of Moringa Oleifera»Biodeterioration of natural and artifical stone»The effect of crude avocado and pawpaw leaves extract on kidney function of guinea pigs»Isolation and characterization of fungi associated with the spoilage of corn grown in farms in Rukpokwu community»Microbial examination of raw meat obtained at main abattoir, Offa»Isolation and identification of bacterial aerosols bioaerosols dump sites in Yenagoa»Incidence of staphylococcus and salmonella species in Kunuaya retailed in Yenagoa